Several studies have shown relationship of biometric testicular, hormonal profiles and fertility. Objective of present research were to predict the fertility of stallion by biometric testicular and hormonal analysis. Measurement of the testis of adults Arabian stallions (n=36) from National Haras Chaouchoua of Tiaret, Algeria. The length (TL), the width (TW), the height (TH), and the scrotal width of the testicles (TSW) during three consecutive seasons (April: 2014-2016). From these measurements, the total testicular volume (TTV), the daily sperm output (DSO) and the daily sperm product (DSP) were calculated. The serum testosterone concentration, the follicle-stimulating hormone (FSH) and the luteinizing hormone (LH) were also evaluated by IMMUNOTECH® during April 2016.The scrotal width (TSW: 11.06 ± 1.55) cm, was very highly correlated to the age and the body weight of the stallions as well as with all the testicular parameters (p < 0.01). The scrotal width (TSW) was correlated positively to testosterone, (r=0,14).The examination of the testicular size is an important criteria to estimate for selection the stallions with highest fertility potential.