To study the effect of several environment-friendly materials on total phenols of two varieties of squash, field experiments were carried out on squash (Cucurbitapepo L.) cv. Maharaja and Eskandarani during 2015, early summer, summer and nili cultivations. The results indicated that in cultivar Maharaja, the different treatments reduced the total phenol contents of leaves as following order, a mixture oil of camphor + ginger (78.19%) > ginger oil (58.78%) > methomyl (54.02%) > a mixture of camphor oil + lequrice root extract (50.12%) > spinetoram (45.16%) > a mixture of ginger oil + lequrice root extract  (35.49%) > camphor oil (33.91%) > lequrice root extract (31.17%) > azadirachtin (29.15%) > spinosad (25.63%), whereas in  Eskandarani cultivar this reduction order was ginger oil (55.36%) > methomyl (55.25%) > azadirachtin (51.30%) > a mixture oil of camphor + ginger (40.40%) > a mixture of ginger oil + lequrice root extract  (37.07%) > camphor oil (34.69%) > a mixture of camphor oil + lequrice root extract (29.96%) > spinosad (29.24%) > spinetoram (27.77%) > lequrice root extract (17.83%). Moreover, it was observed fluctuations in total phenol contents of squash leaves among its three cultivations, early summer, summer and nili when treated with varied eco-friendly materials as well as between the two cultivars studied. It was concluded that the environment-friendly materials have a negative effect on total phenol contents of squash leaves and the interaction among cultivars of squash, season of cultivations and environment-friendly materials plays a great role on this effect.