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185533

Mechanism of antimicrobial activity and antioxidant activities of the essential oil and the methanolic extract of carum montanum from Algeria

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

Microbiology

Abstract

The methanolic extract (ME) of C. montanum obtained by a hydo-alcoholic maceration and its polyphenol content was evaluated by Folin-Ciocalteu method. This extract and C. montanum essential oil (EO)were screened for antimicrobial activity against 21 microbial strains by agar diffusion method. MICs of the EO were determined by the broth micro dilution method. The action mechanism of EO was determined on the susceptible strains by the time kill assay and the lysis experience. Antioxidant properties were studied by both free DPPH radical scavenging and reducing power techniques. The TPC in the ME showed a high level of 101.50 ± 5.33 mg GAE /mg. B. cereus was the most sensitive strain with MIC of 55.5 µg/ml , then K. pneumoniae (111 µg/ml). A remarkable decrease in a survival rate as well as in the absorbance at 260 nm were recorded, which suggest that the cytoplasm membrane is one of the targets of the EO. Antioxidant effects concentration were dependent and IC50 values were 1.09 ± 0.37 µg/ml for the EO and 65.04 ± 0.00 µg/ml for the ME by DPPH method and a reducing power dose-dependent. In conclusion, C. montanum extracts showed potent which could be exploited in the food industry for food preservation.

DOI

10.21608/assjm.2018.185533

Keywords

C. montanum, Apiaceae, Essential oils, antimicrobial activity, Antioxidant activity, Reducing power

Authors

First Name

MERIEM

Last Name

EL KOLLI

MiddleName

-

Affiliation

Laboratory of Natural Biological Resources, University of Sétif 1, Sétif, Algeria.

Email

elkollim@yahoo.fr

City

-

Orcid

-

Volume

56

Article Issue

4th ICBAA

Related Issue

8694

Issue Date

2018-04-01

Receive Date

2021-07-26

Publish Date

2018-04-01

Page Start

253

Page End

262

Print ISSN

1110-0419

Online ISSN

2974-4830

Link

https://assjm.journals.ekb.eg/article_185533.html

Detail API

https://assjm.journals.ekb.eg/service?article_code=185533

Order

47

Type

Original Article

Type Code

841

Publication Type

Journal

Publication Title

Annals of Agricultural Science, Moshtohor

Publication Link

https://assjm.journals.ekb.eg/

MainTitle

Mechanism of antimicrobial activity and antioxidant activities of the essential oil and the methanolic extract of carum montanum from Algeria

Details

Type

Article

Created At

22 Jan 2023