This study was an attempt to develop an in vitro protocol for propagation date palm(Phoenix dactylifera L.) Zaghlol cv via direct shoot regeneration system. Combinations between plant growth regulators were used in order to achieve this goal. Shoot tips were cultured on MS medium supplemented with cytokinins namely BA or KIN at different concentrations either individually or in combinations with NAA at 0.0, 1.0, 2.0 and 4.0 mg /L in the presence of 1.0 mg/ L NOA. Results revealed that MS medium supplemented with BA or KIN at 1.0 mg /Lin combination with NAA at 1.0 mg/ L was the best for bud formation from shoot tip after 8 weeks of culturing at initiation stage. Sub-culturing the formed buds on solid MS medium supplemented with 1.0 mg/ L of BA and KIN gave the maximum number of shoots with appropriate length at multiplication stage. MS medium supplemented with 10 mg/ L of GA₃ caused the maximum shoot length at elongation stage compared with control and other treatments. Moreover, maximum rooting percentage, root number and root length were obtained as MS medium supplemented with 0.5 mg/L IBA and 1.0 mg/L NAA was used.