The development and implementation of a biosecurity plan in a poultry production are the main cause for its success. So, this study was carried out to monitor and evaluate the hygienic level of different poultry farms according to aerobic plate count( APC) and isolation of some hygiene indicator bacteria including Staphylococcus spp., Streptococcus spp. and Pseudomonas spp. A total of 2160 environmental and bird samples as well as swabs were collected from broiler chicken, layer chicken, breeder chicken and duck farms. Our results showed that, there is a negative relationship between the biosecurity level and the hygiene indicator bacteria. The APC was mainly high in the environmental samples specially with low biosecurity levels. The highest prevalence of Staphylococcus spp., Streptococcus spp. and Pseudomonas spp. was 86.11 %, 70 % and 83.33 % of layer farm B, duck farm A and layer farm B, respectively while the lowest prevalence of Staphylococcus spp., Streptococcus spp. and Pseudomonas spp. was 52.22 %, 34.45 % and 32.22 % of breeder farm B, breeder farm A and broiler farm C, respectively. Finally, a good applied biosecurity strategy is the first way to protect poultry from bacterial colonization. As always, prevention is better than control, and investment in biosecurity and hygiene are the best ways for success stories in poultry production.