A total of 300 samples from apparently healthy chickens were collected in this study.This samples represented as 100 samples from trachea, 100 samples from lung and 100 samples from heart. They were subjected to morphological and biochemical examination for identification of P.Multocida.An incidence of P. multocida by cultural examination was 21 (7%);- 0% from tracheal swabs, 13% from lung and 8% from heart while an incidence by biochemical examination was 6 (2%) ;- 0% from tracheal swabs - 3% from lung and 3% from heart. All suspected P. multocida colonies were subjected to PCR for identification of P. multocida isolates and proved that six isolates were found to be positive for P.multocida with an incidence of 2% which similar to the results of confirmatory biochemical examination.The drug sensitivity test mentioned that P. multocida was sensitive to ciprofloxacin and norfloxacin.Six suspected isolates of P.multocida identified by cultural examination were tested by polymerase chain reaction method by using specific primers for P.multocida . The stn gene was amplified in four isolates giving 617bp, while the InvA gene was amplified in four isolates giving 284bp.