The purposes of this practical research to detect the incidence of pathogenic E. coli in kariesh, domiatti and tallaga cheeses. Confirmative serological identifications of the isolated strains of pathogenic E. coli were performed. One hundred and fifty random samples of kariesh, domiatti and tallaga cheeses (50 each) were collected from different shops and street vendors in El Gharbia territories. Samples were bacteriologically examined for the presence of E. coli. The incidence of E. coli in the analyzed samples was 54%, 42%, and 32%, respectively. Serotyping of isolated pathogenic serovars of E. coli were: O119, O111, O86 & O128, and O124 in domiatti cheese. Genotypic identification of E. coli virulence genes (Stx1, eaeA, fimH) were recorded at percentage of Zero %, 28.5% and 100 % respectively. In kariesh cheese. the detected E. coli in the analyzed samples surely able to causes public health disease outbreaks to the consumers and stipulated the local hygienists to insures maximum health's stipulations.
Therefore this work was planned to covers the followings points:1-Isolations, biochemical and serological identifications of pathological Escherichia coli from kariesh, tallage, and domiatti types of cheeses in El _Gharbia governorate. 2-Molecular identifications of isolated bacteria (bacterium coli) using the PCR technique.