Objective of the present study was to isolate, identify and genetically characterize bovine herpes virus Type 1 (BoHV-1) from suspected cattle showing clinical symptoms of respiratory disease in Dakahlia, Egypt, September 2018.The virus from suspected nasal swabs and lung tissue samples was adapted onto specific pathogen free - embryonated chicken eggs (SPF - ECE).Pock lesions on the chorio-allantoic membrane (CAM) of SPF - ECE ranged from 1 to 2 mm in diameter and scattered all over the membrane that were more pronounced after the third passge. Sensitive PCR assay specific for BoHV-1 gC gene was also performed for molecular identification of the viral isolate and the gC PCR product was sequenced. Phylogenetic analysis of gC gene sequence identified and clustered our isolate with BoHV1.1. This highlights the isolation, rapid and sensitive detection of BoHV-1 virus from clinical cases and its subtyping by nucleotide sequencing and subsequent phylogenetic analysis which gives valuable information about the molecular epidemiology of BoHV-1 subtypes prevalent in the country.