The current study aimed to investigate the protective effect of the thymol (20 mg/kg BW) or carvacrol (15mg/kg BW) on gentamycin (100mg/kg) induced oxidative stress in rat. Thymol and carvacrol were administrated orally once daily for 12 consecutive days. After one week of acclimatization, 30 adult male albino rats weighting 150-200g were used for the experiment. They were allocated into 5 equal groups (n=6). group1 (control), group2 (tween), group3 (gentamycin), group4 (thymol+gentamicin), and group5 (carvacrol+gentamcin). At the end of experiment, Rats were euthanized humanly and blood samples were collected to study the nephro, hepatic and inflammation markers. The findings revealed that there were significant decrease in serum blood urea nitrogen, creatinine and MDA in thymol and carvacrol groups but there were significant elevation in renal SOD, CAT and GPX. There were significant decreases in ALT, ALP, AST and GCT in thymol and carvacrol groups. Additionally, CRP, IL6 and TNF –α were decreased in thymol and carvacrol gps. The result revealed that each of the thymol and carvacrol has antioxidant and protective effects against the hepatic toxicity and nephrotoxicity induced by gentamicin in rats.