This study was conducted to evaluate the therapeutic efficacy of probiotics (Lactobcillus acidophilus, Lactobacillus planterum and Bifidobacterium bifidum) and ruminal juice transplantation in induced lactic acidosis in sheep. Ten sheep were used in this study in addition ten apparently healthy sheep were used as a control. The sheep with induced acidosis showed inappetance, weakness, depression, tympany, staggering movement and diarrhea. There was significant increase (p<0.05) in respiratory rate and pulse rate, while body temperature and ruminal movement were significantly decreased (p<0.05). There were significant increase (p<0.05) in Hb content, PCV%, RBCs, WBCs, granulocyte, lymphocyte and monocyte in inducd acidotic sheep than healthy one. Biochemically, there was significant decrease (p<0.05) in Na, Cl, Mg, Ca, SOD, catalase, albumin, total protein and vitamin B12; whereas significant increase (p<0.05) in K, P, AST, ALT, GGT, ALP, urea, creatinine, MDA, CRP and histamine were detected in induced acidotic sheep. The clinical, hematological, biochemical and ruminal parameters were significantly changed toward the control values after treatment with of probiotics and ruminal juice transplantation from healthy sheep. Therefore, probiotics and ruminal juice transplantation are recommended for treatment of sheep suffered from acidosis.