Enterococcus species are considered as a major etiological agent of nosocomial infections, they are commonly isolated from different sources of food. So, this study was conducted for detection of Enterococcus spp., from (10 fish samples, 30 herbs samples, 20 drinking water samples, 30 dairy products samples, and 20 meat products samples) from Cairo Governorate, Egypt. The results revealed that from 110 food samples analyzed 11.8% were positive for Enterococci, E. faecalis was detected by percentage 53.8%. All E. faecalis isolates were resistant to vancomycin (100%), erythromycin (57.1%), ciprofloxacin (14.3%), chloramphenicol (42.9%), tetracycline (57.1%), while none of the E. faecalis isolates were resistant to ampicillin, penicillin or norfloxacin. PCR was done for detection of some antibiotic resistant genes (mphC, norA, tetK, floR, vanA). Genotypic characterization revealed that tetK and floR genes were present in the all 7 E. faecalis isolates. While, vanA gene was detected in 3 isolates, and mphC gene was detected only in 2 isolates. The results of our investigation indicated high levels of contamination with multi resistant E. faecalis strains of serious concern with the isolation of strains resistant to vancomycin, considering that vancomycin is the alternative agent for patients who are intolerant to penicillin or who have Enterococci infections with high level resistance to penicillin.