The current study was aimed to investigate the incidence and bacteriological characterization of Salmonella serovars in laying ducks. A total of 52 samples of laying ducks were collected from different farms in Kalubia governorate, Egypt. Samples were isolated from liver, spleen, ovaries, oviducts and intestine. Salmonella isolation revealed a total percentage of 2%; ovaries & oviducts revealed a high incidence among the examined samples (4%), followed by liver & intestine with incidence of 3 and 1%, respectively. The results revealed one isolate of Salmonella strain which subjected to biochemical and serological identification. The isolated Salmonella was identified as being a non-lactose fermenting, (NLFs) Gram negative rod-shaped organism, oxidase negative, catalase positive, indole and Voges Proskauer (VP) negative, methyl red and Simmons citrate positive, H2S producing and urea negative. The isolated Salmonella serotyped as S. Typhimurium O1, 4, [5], 12: i : 1, 2 at Serological Department of Animal Health Research Center. Antimicrobial sensitivity test was conducted on the isolated Salmonella Typhimurium which showed resistance to doxycycline, ampicillin, gentamycin, colistin, vancomycin and neomycin