Breast cancer is the second leading cause of cancer death in women in the world .The chemopreventive effect of Lysine-Cetrimonium Zinc Complex with zinc oxide nanoparticle and sodium ascorbate (L CZN) on 7,12-Dimethylbenz[a]anthracene(DMBA) induced mammary carcinogenesis was investigated in(60) female rats aged from (4-5)weeks and weighting from (90- 110) gr . Oral dose of DMBA (100mg/k.gr.b.wt).  induced a significant increase in serum ALT, AST, MDA, urea, Creatinine,  and CD20 in serum or tissue .However were observed in DMBA induced mammary cancer in rats depletion in CAT,GSH  and Annexin V were markedly decreased . Administration of (NNC) Novel Nano composite was able to mitigate mammary carcinogenic damage induced by DMBA as to directory pronounce chemo preventive effect against lipid peroxidation, sever enzymatic changes  and maintained glutathione status and antioxidant enzymes in addition histopathological changes directed towards  control. It could be conducted that  administration of (NNC) have potential to exert therapeutic effect against mammary carcinogenesis