The study aims to assess the relation between oxidative stress, antioxidant activity and postparturient hemoglobinuria on70 hemoglobinuria and 30 apparently healthy buffaloes. Blood and serum sampleswere collected and tested for various parameters of oxidative stress and antioxidant enzymes. Significant increase in erythrocytic MDA (malondialdehyde) whereas significant decrease in serum inorganic phosphorus, copper (Cu), selenium (Se),erythrocytic SOD (superoxide dismutase), GPX (glutathione peroxidase) and G6PD (glucose-6-phosphate dehydrogenase). According to these results, there was increase in oxidative stress indicator while antioxidant enzymes activities dramatically decreased in postparturient hemoglobinuria (PPH) affected buffaloes. It was concluded that there is relation between oxidative stress, antioxidant activity and PPH in buffaloes.