The present work investigated the susceptibility of goslings to infection with Duck Hepatitis Virus type-1 (DHV-1) and their possible role for hazard spread of infection to ducklings. It was noticed that experimentally infected one-week old goslings were susceptible to infection with DHV-1 showing clinical signs started by the 4^th day post infection detected in 4 out 10 goslings represented by dullness and weakness. Two goslings were recovered, and other 2 birds were died one-week post infection. In contact ducklings showed classical signs of DHV-1 infection represented by weakness and sudden death. DHV-1 was recovered from liver, spleen and kidneys of experimentally dead birds. Survived goslings exhibited detectable neutralizing antibody titers by the 1^st week post infection. Vaccination of goslings with the live attenuated DHV-1 vaccine resulted in good immune response of high antibody levels up to 10 weeks post vaccination. So, it could be said that goslings were susceptible to DHV-1 and may play a role in transmission of DHV-1 to duckling and it could be recommended to vaccinate gosling against DHV-1 infection