Colchicine is an alkaloid that has been widely used for treatment of gout. It also has a curative effect on cancer, as many studies have shown that its effect on cell apoptosis .The objective of the present study was to evaluate the Biochemical Effect of Cholchicine on Experimentally Induced Leukemia in Rats. Sixty white albino male rats of 8-10 weeks old and 150-200 g weight were ued in the experiment.Rats were randomly divided into six groups 1^st group act as normal control , 2^nd group was injected intraperitoneally with colchicine (0.14mg/kg/wt) one dose daily for four weeks and act as positive control , 3^rd group was administrated orally with benzene (1mg /kg /wt) one dose daily four weeks for induction of leukemia and act as leukemic group , last three groups (4^th , 5^th ,6^th ) administrated benzene at a dose of (1mg /kg /wt) daily and treated with colchicine with different concentration (0.07mg/kg/wt)(0.14mg/kg/wt) and (0.21mg/kg/wt) respectively. Blood samples were collected twice after 2 and 4 weeks for biochemical examination. Intraperitoneal injection of colchicine caused increased in Hb level, platelets count and Caspase- 3 and decreased in Total leucocyte count (WBCs),L.malondialdehyde(L-MDA), Super Oxide Dismutase (SOD) ,Catalase (CAT), Glutathione peroxidase (GPx) , Glutathione (GSH),Alpha –fetoprotein (AFP) ,Interleukin-2 (IL-2),Interleukin-6 (IL-6),Tumor necrosis factor (TNFα), compared with diseased group