A number of 271 apparently healthy camels of both sexes at different ages were used in this study during a period from April 2016 to May 2017. Samples (271 serum samples, 30 milk samples , 21 tissue specimen and lymph nodes) were obtained from slaughtered camels in different localities (Cairo, Giza, El Sharkyia, El Behira, Matroh). Three serological tests were applied including Buffered Acidified Plate Test (BAPT), modified Rose Bengal Plate Test (mRBPT), and Tube Agglutination Test (TAT). The prevalence of reactors for brucellosis was 9.5%, 8.8% and 7.7% respectively. All samples confirmed by using Rivanol Test (Riv.T) and Immunochromatographic assay (ICA) with 8.5% and 9.2% prevalence respectively. All collected milk samples were Negative by MRT (0/30). ICA or (LFA) is highly sensitive, accurate and specific diagnostic assay since it directly detects antibodies of Brucella organism and is considered as rapid confirmatory test.
Culturing from 21 tissue samples and L.N revealed positive isolates as 5/21 (23.8%). The isolated strain was identified biochemically and by Polymerase Chain Reaction (PCR) with 498 bp and revealed predominance of Brucella abortus in all isolates as 5 (100%). This work aims at providing an overview on diagnostic investigations, as brucellosis has an economic impact on the production and reproduction in camels.