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71212

Detection of Staphylococcus aureus and enterotoxin genes from meat products by using conventional and modern identification methods

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Meat hygiene

Abstract

One hundred meat product samples (minced meat, kofta, burger and sausage, 25 of each) were collected and examined for bacteriological evaluation and detection of Staphylococcus aureus by conventional bacteriological methods and by polymerase chain reaction PCR. The mean value of S. aureus counts cfu/g were 0.63×102 ±0.19×102, 0.17×102 ±0.06×102, 0.96×102 ±0.24×102 and 1.1×102 ±0.15×102, respectively. Concerning S.aureus, bacteriological results revealed that the prevalence in minced meat, kofta, burger and sausage was 16%, 12%, 16%, 20%, respectively. In addition, 5 samples out of 100 ones were unaccepted as they were exceeded the permissible limit of E.O.S 2005. Moreover, four random S. aureus samples (2 positive and 2 negative) were reexamined by polymerase chain reaction (PCR) and just one sample was negative by the bacteriological method for S. aureus showed positive results with PCR (false negative sample). Multiplex Polymerase Chain Reaction (m-PCR) was applied for detection of genes responsible for enterotoxins production (sea, seb, sec, sed and see) from identified coagulase positive Staphylococcus aureus. They were detected in the examined minced meat and sausage samples.

DOI

10.21608/bvmj.2019.12556.1009

Keywords

meat products, S. aureus, clfA, enterotoxin, m-PCR

Authors

First Name

Ola

Last Name

Younes

MiddleName

-

Affiliation

Research assistant at Animal Health research institute

Email

vetolafathy@gmail.com

City

banha

Orcid

-

First Name

Hemmat

Last Name

Ibrahim

MiddleName

-

Affiliation

Professor of food Hygiene and Control Department (Meat Hygiene), Fac. Vet. Med. Benha Univ

Email

drhemmatmostafa@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Hassan

MiddleName

-

Affiliation

Professor of food Hygiene and Control Department (Meat Hygiene), Fac. Vet. Med. Benha Univ

Email

mohamedhsn40@yahoo.com

City

Tanta

Orcid

-

First Name

Reham

Last Name

Amin

MiddleName

-

Affiliation

Professor of food Hygiene and Control Department (Meat Hygiene), Fac. Vet. Med. Benha Univ

Email

rehamnour2007@yahoo.com

City

Benha

Orcid

-

First Name

Ahmed

Last Name

maarouf

MiddleName

Afifi

Affiliation

Chief Researcher of Microbiology and Director of Animal Health Research Institute, Benha Branch

Email

drahmed.maarouf@yahoo.com

City

-

Orcid

-

Volume

36

Article Issue

2

Related Issue

5670

Issue Date

2019-06-01

Receive Date

2019-06-26

Publish Date

2019-06-01

Page Start

229

Page End

237

Print ISSN

1110-6581

Link

https://bvmj.journals.ekb.eg/article_71212.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=71212

Order

22

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023