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Detection of virulence genes of enterohaemorrhagic E. Coli isolated from some meat products by polymerase chain reaction

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Last updated: 22 Jan 2023

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Abstract

A grand total of 105 meat product samples of minced meat, sausage and luncheon (35 of each) were
duplicated bacteriologically examined to detect Enterohaemorrhagic E.coli prevalence and some virulence
genes. One replicate was processed for EHEC non O157 by using conventional method for isolation and
identification of E.coli and the other for E.coli O157:H7, then serological typing and PCR technique for
specific stx1, stx2, cvcC and hlyA genes from 6 random samples were applied. E.coli was isolated from 12
samples (34%), 9 samples (25.7%) and 11 samples (31%) of the examined minced meat, sausage and
luncheon, respectively. The isolated serotypes of EHEC were O26 (5 strains) 15.6%, O111 (3 strains) 9.4%
and O157 (3 strains) 17.6%. The incidence of EHEC O26 were (2 strains) 5.7%, (2 strains) 5.7%, (1strain)
2.85%, incidence of O157:H7 were (2 strain) 5.7%, (1 strain) 2.85%, 0% in minced meat, sausage and
luncheon, respectively. The incidence of O111 was 2.85% from each the type meat products. PCR results
indicated that stx2 and cvaC virulence genes were detected in the same studied strain (O157:H7 from minced
meat sample), while stx1 and hIyA genes were not detected. Accordingly, meat products may constitute an
important reservoir for EHEC and PCR technique is the most sensitive and efficient approach for detection
of EHEC genes.

DOI

10.21608/bvmj.2015.31790

Keywords

Enterohaemorrhagic E.coli, Shigatoxins, PCR

Authors

First Name

Ashraf

Last Name

Abd El Tawab

MiddleName

A.

Affiliation

Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University

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Orcid

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First Name

Fatma

Last Name

El-Hofy

MiddleName

I.

Affiliation

Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University

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City

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Orcid

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First Name

Shaimaa

Last Name

Nada

MiddleName

M.

Affiliation

Animal Health Research “Shebin El- Kom branch"

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City

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Orcid

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First Name

Rasha

Last Name

Deiab

MiddleName

A. A.

Affiliation

Animal Health Research “Shebin El- Kom branch"

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Orcid

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Volume

29

Article Issue

1

Related Issue

5398

Issue Date

2015-09-01

Receive Date

2019-05-15

Publish Date

2015-09-01

Page Start

45

Page End

52

Print ISSN

1110-6581

Link

https://bvmj.journals.ekb.eg/article_31790.html

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https://bvmj.journals.ekb.eg/service?article_code=31790

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7

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

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Article

Created At

22 Jan 2023