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31402

Detection of some foodborne pathogens in meat products by Polymerase Chain Reaction

Article

Last updated: 22 Jan 2023

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Abstract

A total of 100 random samples of meat products including raw minced meat, raw sausage, luncheon and
basterma (25 samples of each) were collected from different markets in Cairo and Giza governorates to
be examined bacteriologically for detection of Staphylococcus aureus, Listeria monocytogenes and
Escherichia coli. These samples were examined for isolation of such pathogens by conventional
bacteriological methods and by polymerase chain reaction (PCR). Concerning S.aureus bacteriological
results revealed the prevalence in minced meat, Sausage, luncheon and basterma was (24%, 24%, 20%,
4%) respectively. While L. monocytogenes revealed the prevalence in minced meat, sausage, luncheon
and basterma was (4%, 0%, 0%, 0%) respectively and E. coli revealed the prevalence in minced meat,
sausage, luncheon and basterma was (20%, 20%, 24%, 20%) respectively. The results cleared that PCR
is an ideal method for identification of foodborne pathogens, as it was effective, less labor, more
sensitive, reduces effort and time after using gradient PCR in validation of each microbe.

DOI

10.21608/bvmj.2016.31402

Keywords

Foodborne pathogens, Optimization, polymerase chain reaction (PCR), bacteriological isolation, meat products

Authors

First Name

George

Last Name

Armany

MiddleName

A.

Affiliation

Genome Research Unit., Animal Health Research Institute, Dokki

Email

-

City

-

Orcid

-

First Name

Hanaa

Last Name

Ahmed

MiddleName

A.

Affiliation

Genome Research Unit., Animal Health Research Institute, Dokki

Email

-

City

-

Orcid

-

First Name

Hemmat

Last Name

Ibrahim

MiddleName

M.

Affiliation

Food hygiene Dept., Faculty of Veterinary Medicine, Benha University

Email

drhemmat01@yahoo.com

City

-

Orcid

-

First Name

Reham

Last Name

Amin

MiddleName

A.

Affiliation

Food hygiene Dept., Faculty of Veterinary Medicine, Benha University

Email

rehamnour2007@yahoo.com

City

-

Orcid

-

Volume

30

Article Issue

1

Related Issue

5349

Issue Date

2016-03-01

Receive Date

2019-05-09

Publish Date

2016-03-01

Page Start

323

Page End

330

Print ISSN

1110-6581

Link

https://bvmj.journals.ekb.eg/article_31402.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=31402

Order

36

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

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Details

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Article

Created At

22 Jan 2023