Aiming to raise the levels of immunity induced in dogs by the cell culture inactivated rabies vaccine, bee venom BV was
subjected to investigate its immune stimulant effect in vaccinated dogs. It was found that a dose of 1mg of BV/ dog did
not cause any post inoculation reaction showing its safety. Mutual vaccination of dogs with BV inoculation was carried
out in different groups of susceptible dogs of about 3-5 months' age. Monitoring of the exhibited rabies antibodies in
vaccinated dogs using serum neutralization test (SNT) and indirect Enzyme Linked Immune Sorbent Assay (ELISA)
revealed that BV induced the highest levels of antibodies (128 by SNT and 7 log2 and 6 log2 by ELISA) when inoculated
before and simultaneously with rabies vaccine. Rabies vaccine alone or before inoculation of BV induced lower titers of
antibodies (32&64 and 5 & 6log2 by SNT and ELISA respectively) by the 4th week post vaccination. However, BV could
be used to initiate the immune response of dogs to rabies vaccine.