In this research Romanian Sheep pox virus was identified and confirmed by using PCR, Live attenuated Romanian Sheep
pox vaccine (RSPV) was prepared and its titer on VERO cell was log 10^2.5TCID₅₀/dose. It was sterile, safe and potent.
We used eight susceptible calves 6-8 months old, five calves vaccinated with 0.5 ml of prepared RSP vaccine intra
dermally (I/D) in tail folds while three calves kept as control. Evaluation of both acquired humoral and cellular immunity
by using lymphocyte proliferation assay, SNT, ELISA and Interferon Gamma Bioassay (IFN-ᵧ). The result showed that
lymphocyte proliferation began to increase till reach to its peak (1.629) at 10th day then decrease after that, While
Interferon gamma (IFN-ᵧ) detected in 1^st day till 7^th day post vaccination then decreases after that. Results of SNT and
ELISA revealed that Protective serum neutralizing antibody titer started at three weeks (1.5), (1.09) post vaccination then
reach to its peak at 12^th weeks (2.5), (1.85) respectively and persisted till 28 weeks. From this study we found that live
attenuated RSP vaccine a good immunogenic response where it was induced a high level of antibody titer with prolonged
duration of immunity and higher lymphocyte and interferon gamma levels, So It considered good vaccine to control
Lumpy Skin Disease (LSD)