The present study was performed to evaluate the efficiency of disinfection process applied in poultry farms and
determination the major input of infection into poultry farms. A total of 140 samples and swabs were collected from farm
A and B with an average of 70 samples from each farm. These samples were collected from floor, walls, drinker, feeder,
air, windows, fans, boats, car rubber, cloaca, litter, water, feed and hands samples (5 of each) from two broiler farms
before and after disinfection. The results showed that Salmonella Kentuckey, Salmonella Typhymurium, Salmonella
Enteritidis and Salmonella Virchow were higher in different samples taken from farm (A) than in different samples taken
from farm (B). Actually, E. coli serotype O1 was detected in drinker, boots, cloaca, litter (20% of each) & car rubber
(40%) of farm (A) and only from car rubber (20%) of farm (B). Moreover, E. coli serotype O2 was isolated from fan,
feeder, feed (20% of each) & boots (40%) of farm (B). However, E. coli serotype O78 was detected in floor, feeder,
cloaca, water (20% of each) & feeder & litter (40% of each) of farm (A) and feeder, cloaca (20% of each) & litter (40%)
of farm (B). In concern to S. aureus, the isolation percentages from wall, fan, cloaca, litter (20% of each), floor, drinker,
boots, air (40% of each), car rubber (60%) & worker hands (80%) of farm (A) and only from drinker & car rubber (20%
of each) of farm (B). While, C. jejuni was recovered floor, wall, fan, feeder, boots, water (20% of each), drinker, cloaca
(40% of each) & litter (60%) of farm (A) and only from cloaca and litter (20% of each) of farm (B).