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31236

Molecular identification of M. bovis BCG by Multiplex PCR

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Last updated: 04 Jan 2025

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Abstract

Mycobacterium bovis (M. bovis) BCG belongs to Mycobacterium tuberculosis complex (MTBC), highly related
organisms, which are 99.9 % similar at nucleotide level and phenotypically similar. Its differentiation from other members
of MTBC by conventional methods is laborious and time consuming. PCR provides a rapid alternative method for
differentiation between members of MTBC. It depends on identification of region of difference (RD) which have been
lost during attenuation of M. bovis. Two different BCG strains (from two sources) were confirmed as a member of M.
tuberculosis (MTB) complex (MTC) and as BCG strains by PCR using primers to a region of the 16S rRNA gene that is
conserved in all mycobacteria and region of difference (RD1, RD4, RD9 and RD12) respectively. DNA of Mycobacterium
tuberculosis was used as a control to compare with BCG strains. The results showed that 16S rRNA gene was present in
all tested strains, while the RD1, RD4, RD9 and RD12 were absent only in BCG strains

DOI

10.21608/bvmj.2016.31236

Keywords

Region of difference, BCG, PCR

Authors

First Name

Ashraf

Last Name

Abd El-Tawab

MiddleName

A.

Affiliation

Department of Bacteriology, Immunology and Mycology Fac. Vet. Med. Benha University, Egypt

Email

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City

-

Orcid

-

First Name

Fatma

Last Name

El-Hofy

MiddleName

I.

Affiliation

Department of Bacteriology, Immunology and Mycology Fac. Vet. Med. Benha University, Egypt

Email

-

City

-

Orcid

-

First Name

Essam

Last Name

Nasr

MiddleName

A.

Affiliation

Department of diagnostic bacteriology. Veterinary serums and vaccines research Institute, Abbasia, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Nammalwar

Last Name

Sriranganathan

MiddleName

-

Affiliation

Department of Biomedical Sciences & Pathobiology, Virginia-Maryland College of Veterinary Medicine, USA

Email

-

City

-

Orcid

-

First Name

Enas

Last Name

Soliman

MiddleName

A.

Affiliation

Department of Bacteriology, Immunology and Mycology Fac. Vet. Med. Benha University, Egypt

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-

City

-

Orcid

-

Volume

31

Article Issue

1

Related Issue

5340

Issue Date

2016-09-01

Receive Date

2019-05-07

Publish Date

2016-09-01

Page Start

119

Page End

123

Print ISSN

1110-6581

Article File

BVMJ_Volume 31_Issue 1_Pages 119-123.pdf

PDF . 345.7kB

Link

https://bvmj.journals.ekb.eg/article_31236.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=31236

Order

19

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

Molecular identification of M. bovis BCG by Multiplex PCR

Details

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Article

Created At

22 Jan 2023