The aim of this study was to demonstrate the antioxidant effect of selenium in its ordinary and Nano form on oxidative
stress induced by iron overload in male rat. This study was carried out on 90 rat, weighted 180-200 gm. Rats were
classified into six groups Group 1: control group. Group 2: Nano selenium-treated group. Group 3: selenium treated
group. Group 4: iron overload treated group. Group 5: iron overload + Nano selenium - treatment. Group 6: iron overload
+ selenium-treatment. Blood and liver tissue samples were collected from all groups after 1, 3 and 6 weeks from the onset
of the experiment. Serum used for measurement of total iron. Liver tissue was processed for measurement of
molonadialdhyde (MDA), total antioxidant capacity (TAC), glutathione peroxidase (GSH-PX), super oxide dismutase
(SOD), glutathione reduced (GSH) and Catalase. The obtained results revealed that, Nano-selenium + iron and selenium
+ iron-treated groups lead to decrease the concentration of MDA, total iron and significant decrease in GSH-PX-1 mRNA
expression and increase the concentration of TAC, catalase, GSH and SOD when compared with iron overload treated
group. From the result, it can be concluded that selenium in its ordinary and Nano forms have antioxidant and
hepatoprotective effect in experimentally-induced iron overload in male Wister rats. Nano form has superior effects than
ordinary form of selenium