In the present study an inactivated tissue culture adapted RVF virus vaccine was prepared using
Montanide oil IMS 1313 nanoparticles as adjuvant then evaluated in sheep in comparison to the local RVF
virus vaccine with aluminum hydroxide gel adjuvant using serum neutralization test (SNT) and Enzyme
Linked Immuno-Sorbent Assay (ELISA). The prepared vaccine was sterile and safe inducing no systemic or
local clinical signs in sheep. Results indicated that the prepared nanoparticles oil based vaccine induced a
protective neutralizing serum antibody titer from the 2^nd week post vaccination (WPV), reached the highest
level at the 3^rd month post vaccination (MPV) and persisted in protective level until the for 9^th MPV, while
aluminum hydroxide gel based vaccine appeared in protective level at 2^nd WPV, reached the highest level at
the 2^nd MPV and persisted in protective level till the 7^th MPV. These results were confirmed by using ELISA.
These results demonstrated the potentiality of the prepared nanoparticles oil based vaccine compared to the
locally produced aluminum hydroxide gel based vaccine reflected by greater immunogenicity and longer
duration of immunity.