Present study focused on evaluation of the effect of national nancomposite product on serum biochemical profile and
programmed cell death (apoptosis) by using Annexin V-FITC and PI test of breast carcinoma female rats. Basic nanocurcumin
was tested for its protective effect on breast cancer female rats (n= 30) divided in two main groups (NTBR) and
(TBR) which in turn classified into (TBR- Control Positive and TBR- basic nano- curcumin protective groups). Basic
nano-curcumin was formulated and tested for both IC₅₀ (IC₅₀ = 1.0 μg) and LD_5o (35 mg/kg by wt.) by using in vitro and
in vivo techniques, respectively. Breast cells apoptosis was determined by Annexin V-FITC, which is a fluorescent probe
binds to phosphotidylserine (PS) in a Ca ²⁺ dependent pathway. Breast Cells were stained with Annexin V and PI. The
apoptotic cells [Annexin (+) and PI (-)] were differentiated from necrotic cells [Annexin (-) and PI (+)] using flow
cytometry technique. The anti-proliferative effect of basic nano-curcumin appeared much earlier due to its nano form.
Also, Liver [ALT (40U/l), AST (85 U/l)] and kidney [BUN (19 mg/dl), Creatinine (0.5 mg/dl)] functions were decreased
within normal ranges