Tourists around the world have become more environmentally conscious, and the demand for green accommodation has experienced a rapid growth. In this background, the form of accommodation known as “eco-lodges" have gained a huge popularity, especially among those who are seeking environment friendly wilderness experiences. Extensive studies on characterization of visitors seeking accommodation in eco-lodges have been documented in the tourism literature. However, empirical studies conducted on understanding attitudes, perceptions, characteristics of guests staying in eco-lodges in the Egypt destinations are lacking. A better understanding of such information on tourists is vital in ecotourism planning and development. The purpose of this study was to expand the current literature by determining the demographic characteristics, Accommodation characteristics, and service quality perception differences between each guests group in Eco-lodge guests on Egypt. A self-administrated questionnaire was used to collect data from 202 guests in Eco-lodge on Fayoum and Dahkla. A random sample-sampling plan was used to collect samples. Frequencies, percentages were calculated. Then, one-way analysis of variance (One-Way ANOVA) to determine which group differs significantly from each other the study found that eco-lodge guests were typically highly educated, ages of 25 less than   40, from Egypt and other country who worked full time and had a very high household annual income. On average, guests stayed in Egyptian Eco-lodge for 1–4 days and stayed at the Eco-lodge for three to four nights and visited with their friends and family to learn and explore nature and a new culture. The internet, travel guidebooks and recommendations from friends and relatives were the three most important sources of information that influence on guests' decision making the process to choose the Eco-lodge. Results indicate that the perceived Eco-lodge service quality differs according to the guests' education level and age group. As the eco-lodge business grows and becomes more competitive, managers must learn about and cater to their market group in order to position themselves favourably in the global marketplace.