This study was done to indicate the activity of secondary metabolites of cyanophyta algal extracts for Anabaena Azolla which live in Azolla plant in Egypt  and to study the effective of secondary metabolites in An.Azolla extracts (cold water and methanol extracts)  to inhibition species of  fungi (Fusarium oxysporum, penicillium expansum, Aspergillus niger and candida albicans) also used of HPLC analysis to detected some of active secondary metabolites was done only for methanol extracts also to preliminary statements that used of two extracts of blue green alga Anabaena Azolla. The role of An.Azolla extracts to inhibition growth of these fungi was detected through measured diameter of inhibition zone diameters were, 16.58, 11.42, 11.0 & 11.0 mm in case of Fusarium oxysporum, Penicillium expansum, Aspergillus niger and candida albicans respectively, but aqueous extract give inhibition zone were 18.15, 14.13, 14.0 & 13.0mm respectively. and the results showed these extracts had high correlation ratio and fungi shows high sensitivity to these extract. The mode of mechanism action to inhibition was on fungi cell wall by inhibition active enzymes, excelled treatment for cold water extract . The most important active compounds had been detected in HPLC were phenolic compounds and flavonoids compounds these all compounds detected specially in methanol extract of An .Azolla.  The experiment has been conducted under temperature and artificial illumination at growth room of Soilless Culture Laboratory, Sadat city University, Egypt. The results showed that green fodder can be produced in 8 days using hydroponic technique. Trays treated with Azolla gave more green fresh yield (11.8 kg/tray) than untreated ones (7.2 kg/tray). Adding Azolla increased protein content and the percentages of N, P, K by 28.7% and 0.98, 0.82, 0.70 %, respectively, as compared to no addition of Azolla. Zero disease incidences were achieved with treated by Azolla as compared to 26.0 % with those untreated by Azolla. It could be concluded from this study that adding Azolla to hydroponic barley forage production improved green forage yield and its chemical analysis and free from root rot fungi.