The goal of this study was to investigate the prevalence and antimicrobial resistance of Escherichia coli isolated from 100 samples of raw beef meat (50) and chicken meat (50) Collected From supermarkets at Menofia, Cairo and El-Kalyobia governorates, Egypt. E. coli was recovered from 13 (26%) beef meat samples and 18 (36.00%) chicken meat samples and identified serology. the most common serovars (O26, O119, O111, O103, O91, O86, O146, O45, O121 and O 128). A total of 75 E. coli isolates were obtained and tested for susceptibility to 10 antimicrobials by the disk diffusion method. E. coli isolates of the current investigation revealed a high resistance against Tetracycline (33.3%), Ampicillin (26.6%), Kanamycin & Trimethoprim-Sulphamethoxazole (20%), Cefotaxime (12%), Chloramphenicol & Ciprofloxacin (8%) antimicrobial agents. Whereas; the lowest degree of resistance was noticed against Streptomycin (6.6%), Ceftazidime (5.3%) and Nalidixic acid (4%). These data suggest that antimicrobial-resistant E. coli isolates are widely distributed in meat samples and processing environment in Egypt, which can play a role in dissemination of antimicrobial resistance to other pathogenic and commensal bacteria.