This study was conducted on beef burger, luncheon and sausage (35 of each) collected from different supermarkets at Menoufia Governorates for Detection of virulence genes in isolated  E.coli  and S.aureus using Multiplex PCR of E. coli and S. aureus. The data obtained from this study showed that the incidence of serologically identified E. coli was O₁, O₂₆, O₁₂₅ and O₁₄₂. Multiplex PCR showed Shiga toxin 2 gene (stx2)  detected in O₂₆ and O₁₂₅, while shiga toxin 1 gene (stx1) detected in O1, O₁₄₂ and O₂₆. While O₂₆ Positive E. coli strains for stx1, stx2, eaeA and hylA  genes, O₁, O₁₄₂ Positive E. coli strains for stx1, eaeA and hylA and O₁₂₅ Positive E. coli strains for stx2 and hylA. Regarding to S. aureus was isolated from examined products showed that 7 strains were toxin-producing and the most detected enterotoxin gene in the examined strains was SEA (2 isolates), followed by SEC (3 isolates), then SEB and SEA (1 isolates) and SED and SEA (1 isolates) and 8 strains were negative for 4 enterotoxin-producing genes.