Abstract: T he current research aims to identify the degree of twenty-first century skills application among university students in the Kingdom. By determining the level of skills available in the university curricula from the students' point of view, determining the level of application of the skills among university students and indicating the differences reflected in the response of the sample members (gender and specialization).The research sample consisted of (576) male and female students from Saudi universities. The researcher prepared a scale through reviewing several studies related to its construction and application to use it with the participants. This scale consisted of two parts: The first part is a survey measuring the 21st century skills in the university curricula. The second part is the university students' application of the skills of the twenty-first century.The results of the research showed that the skills of the 21st  century are available and applicable in the university curricula with a high degree among university students, with statistically significant differences between the literary and scientific disciplines of students in favor of the scientific students. There are no differences between the gender of the participants in possessing the skills of the 21st century, except in flexibility in favor of males.