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203035

Biocontrol of Acinetobacter baumannii-S-MH Using Bacteriophages Isolated from Sewage Water

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Last updated: 22 Jan 2023

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Abstract

The aim of this study is to isolate and characterize bacteriophages, as an alternative solution against multidrug-resistant Acinetobacter baumannii-S-MHbacteria causing an outbreak in hospital environments and intensive care units (ICUs). Here, biochemically; isolating and characterizing Acinetobacter baumannii-S-MHby the automated Vitek 2 system. Isolated Acinetobacter baumannii -S-MHbacteria was tested against a collection of Antibiotics by Antibiotic sensitivity test. Acinetobacter baumannii showed resistance to most of the antibiotics tested. To overcome this problem, Acinetobacter baumannii's phage (VB_AciM-AM-M) was isolated, identified, and applied to control the growth of this opportunistic pathogen. Morphologically, phage was identified by TEM; showed that (VB_AciM-AM-M) phage is shaped Myoviridae morphotype. The one-step growth curve of the phage showed burst sizes of 290 PFU/cell with a latent period of 20 minutes for (VB_AciM-AM-M) phage. Complete inhibition of bacterial growth was achieved using phage with MOIs of 1, 3 and 5 after 1, 3, and 24 h. of incubation at 37°C. Hence, this study indicates that the isolated bacteriophages are promising biocontrol agents that could challenge antibiotic-resistant Acinetobacter baumannii-S-MHbacteria to announce new successful alternatives to antibiotics.

DOI

10.21608/eajbsg.2021.203035

Keywords

Acinetobacter baumannii -S-MH, antibiotics, Bacteriophages and Biocontrol

Authors

First Name

Abdel-Monem

Last Name

O.

MiddleName

M.

Affiliation

Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13511, Egypt.

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Orcid

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First Name

Ahmed

Last Name

Esmael

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13511, Egypt.

Email

-

City

-

Orcid

-

First Name

Soheir

Last Name

Abdelrahman

MiddleName

-

Affiliation

Clinical pathology Department, Faculty of Medicine, Benha University, Benha 13511, Egypt.

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City

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Orcid

-

First Name

Amira

Last Name

Shraf

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13511, Egypt.

Email

amira.ashraf7795@gmail.com

City

-

Orcid

-

First Name

Mervat

Last Name

Hassan

MiddleName

G.

Affiliation

Botany and Microbiology Department, Faculty of Science, Benha University, Benha 13511, Egypt.

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Orcid

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Volume

13

Article Issue

2

Related Issue

27165

Issue Date

2021-12-01

Receive Date

2021-09-05

Publish Date

2021-12-01

Page Start

17

Page End

32

Print ISSN

2090-0872

Online ISSN

2090-0880

Article File

EAJBSG_Volume 13_Issue 2_Pages 17-32.pdf

PDF . 1.1MB

Link

https://eajbsg.journals.ekb.eg/article_203035.html

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https://eajbsg.journals.ekb.eg/service?article_code=203035

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Original Article

Type Code

689

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, G. Microbiology

Publication Link

https://eajbsg.journals.ekb.eg/

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Article

Created At

22 Jan 2023