If the micro-organisms, tissues, cells, enzymes, DNA, or peptide used to identify the target, the process is known as a biosensor. HPLC and UV radiation are used for Vitamins measurement which is time-consuming and expensive. So, biosensors can remove the restrictions. In this study, for the first time, the amount of water-soluble vitamins by plant bionanosensor that is a new approach was became to a visible rotation, and its amount can be detected through this rotation. For the detection of vitamin B12 and vitamin C antioxidants in the form of injection ampoules, the rotational effect of them was analyzed by plant bionanosensor in a completely randomized design with three replications by sas9.1 software. 10.5It was determined that plant bionanosensor capability of detecting vitamin B12 and vitamin C through the rotation is likely to be 99%. Plant bionanosensor converts the existence of these two types of vitamins to a meaningful rotation, so its rotation is a criterion for measuring.