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278611

Total Phenolic Content and Antimicrobial Activity of Mentha rotundifolia L. from Western Algeria

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Last updated: 03 Jan 2025

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Abstract

The objective of the present study is to the total phenolic compound determination, as well as the in vitro evaluation of methanolic and ethanolic extracts antimicrobial activity of the medicinal plant Mentha rotundifolia L. against pathogenic strains. The Follin-Cioacalteu method was used to determine the total polyphenols, while the flavonoids were estimated according to the colorimetric method with aluminum chloride. In addition, the determination of condensed tannins is performed by the vanillin method.  The antimicrobial activity is tested on five pathogenic bacterial strains, two of which are Gram-negative (Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853), three Gram-positive (Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923 and Micrococcus leutus ATCC 4698), and one yeast (Candida albicans ATCC 26790), by the diffusion on solid medium and microdilution methods. The ethanolic extract of round leaf mint from the Bechar region is very rich in polyphenols and flavonoids (401.75 ± 0.32 mg GAE/g, 549.75 ± 1.23 mg QE/g) compared to the methanolic and hydroalcoholic extracts of this plant from the Sidi-Bel-Abbes region. The results of the obtained well method showed that all bacterial strains exhibited sensitivity to the ethanolic extract of Mentha rotundifolia L. from the Bechar region, with inhibition zones up to 21,67 ± 0,57 mm and minimum inhibitory concentrations (MIC) varying between 06.25 mg/ml and 100 mg/ml, The effect of the other extracts on the tested strains was less significant, the methanolic extract was the least effective. These results demonstrate that the Mentha rotundifolia L. ethanolic extract has a great potential of secondary metabolites with antimicrobial activity.

DOI

10.21608/eajbsc.2022.278611

Keywords

Mentha rotundifolia L, ethanolic extract, methanolic extract, Phenolic compounds, Pathogenic strains, antimicrobial activity

Authors

First Name

Bouyakoub,

Last Name

Nesrine

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

bou.nesrine@outlook.fr

City

Algeria

Orcid

-

First Name

Kanoun,

Last Name

Khedoudja

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

khedoudja.kanoun@univ-sba.dz

City

Algeria

Orcid

-

First Name

Bouchouicha,

Last Name

Sara

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

sara.bouchouicha@univ-sba.dz

City

Algeria

Orcid

-

First Name

Nourine,

Last Name

Zeyneb

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

-

City

-

Orcid

-

First Name

EL-Kadi,

Last Name

Zohra

MiddleName

Fatima

Affiliation

Valorisation of Phytoresources and Eco-Development of Spaces Laboratory, Environnement Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbès, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

-

City

Algeria

Orcid

-

First Name

Harir,

Last Name

Noria

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

-

City

Algeria

Orcid

-

First Name

Abbouni,

Last Name

Bouziane

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

-

City

Algeria

Orcid

-

First Name

Megharbi,

Last Name

Aicha

MiddleName

-

Affiliation

Valorisation of Phytoresources and Eco-Development of Spaces Laboratory, Environnement Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbès, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

-

City

Algeria

Orcid

-

Volume

14

Article Issue

2

Related Issue

35452

Issue Date

2022-12-01

Receive Date

2022-10-18

Publish Date

2022-12-11

Page Start

427

Page End

438

Print ISSN

2090-0767

Online ISSN

2090-083X

Article File

EAJBSC_Volume 14_Issue 2_Pages 427-438.pdf

PDF . 790.4kB

Link

https://eajbsc.journals.ekb.eg/article_278611.html

Detail API

https://eajbsc.journals.ekb.eg/service?article_code=278611

Order

278,611

Type

Original Article

Type Code

673

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Publication Link

https://eajbsc.journals.ekb.eg/

MainTitle

Total Phenolic Content and Antimicrobial Activity of Mentha rotundifolia L. from Western Algeria

Details

Type

Article

Created At

22 Jan 2023