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278608

Isolation, Screening and Characterization of an Antagonistic Actinobacteria from Algerian Semi-Arid Region.

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Last updated: 03 Jan 2025

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Abstract

Most antibiotics have been isolated from Actinobacteria, with contributions from the Streptomyces and Nocardia genera. Recently, Rhodococci are known to produce a variety of antimicrobial substances. Soil samples were collected from the Bougtob region in El-Bayadh city (34°02'32.7"N 0°05'21.4"E), using the classic method of Pochon and Tardieux 1962. Thirty isolates with the same Nocardiopsis morphology were isolated on 65. GYM Streptomyces medium using dilution techniques, and tested for their ability to produce antimicrobial agents. The morphological and biochemical tests were used to identify thirty isolates of the Nocardiopsis genus. The antimicrobial activity of the compound produced by the Rhodococci strain was tested by the cross-streak assay method, against the Gram-positive bacteria (Bacillus subtilis, Micrococcus luteus and Staphylococcus aureus) and the Gram-negative bacteria (Escherichia, coli). Out of thirty strains, two showed broad-spectrum activity. The strains were identified using various biochemical tests. These results indicate that the Algerian Sahara soil microorganisms could be an interesting source of antibacterial and antifungal bioactive substances.

DOI

10.21608/eajbsc.2022.278608

Keywords

Actinobacteria, Rhodococci, antibiotics, antimicrobial activity, Semi-arid region

Authors

First Name

Bouchouicha,

Last Name

Sara

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

sara.bouchouicha@univ-sba.dz

City

Algeria

Orcid

-

First Name

Kanoun,

Last Name

Khedoudja

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

khedoudja.kanoun@univ-sba.dz

City

-

Orcid

-

First Name

Belaouni,

Last Name

Ahmed

MiddleName

Hadj

Affiliation

Biology of Microbial Systems Laboratory (LBSM), Kouba Normal Higher School (ENS), Algiers, Algeria.

Email

-

City

Algeria

Orcid

-

First Name

Nourine,

Last Name

Zeyneb

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

-

City

-

Orcid

-

First Name

Bouyakoub,

Last Name

Nesrine

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria

Email

-

City

Algeria

Orcid

-

First Name

Chama, Z

Last Name

Zouaouia

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria

Email

-

City

Algeria

Orcid

-

First Name

Benine,

Last Name

Lamine

MiddleName

Mohamed

Affiliation

Synthesis of Environmental Information Laboratory, Djillali Liabes University of Sidi Bel Abbes. Algeria.

Email

-

City

-

Orcid

-

First Name

Mouffok,

Last Name

Benali

MiddleName

-

Affiliation

Synthesis of Environmental Information Laboratory, Djillali Liabes University of Sidi Bel Abbes. Algeria.

Email

-

City

Algeria

Orcid

-

First Name

Harir,

Last Name

Noria

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

-

City

Algeria

Orcid

-

First Name

Abbouni,

Last Name

Bouziane

MiddleName

-

Affiliation

Molecular Microbiology Health and Proteomics Laboratory, Biology Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbes, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

-

City

Algeria

Orcid

-

First Name

Megharbi,

Last Name

Aicha

MiddleName

-

Affiliation

Valorisation of Phytoresources and Eco-Development of Spaces Laboratory, Environnement Department, Natural Sciences and Life Faculty. Djillali Liabes University of Sidi-Bel-Abbès, BP N°. 89 Sidi-Bel-Abbès 22000 Algeria.

Email

-

City

Algeria

Orcid

-

Volume

14

Article Issue

2

Related Issue

35452

Issue Date

2022-12-01

Receive Date

2022-10-17

Publish Date

2022-12-09

Page Start

415

Page End

425

Print ISSN

2090-0767

Online ISSN

2090-083X

Article File

EAJBSC_Volume 14_Issue 2_Pages 415-425.pdf

PDF . 805.9kB

Link

https://eajbsc.journals.ekb.eg/article_278608.html

Detail API

https://eajbsc.journals.ekb.eg/service?article_code=278608

Order

278,608

Type

Original Article

Type Code

673

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Publication Link

https://eajbsc.journals.ekb.eg/

MainTitle

Isolation, Screening and Characterization of an Antagonistic Actinobacteria from Algerian Semi-Arid Region.

Details

Type

Article

Created At

22 Jan 2023