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261502

Molecular Characterization and Evaluation of The Antimicrobial Activity of Phenoloxidase Purified from Spodoptera littoralis against Different Array of Pathogenic Bacteria

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Last updated: 03 Jan 2025

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Abstract

Phenoloxidase (PO) is an important enzyme for various insect processes, such as defensive encapsulation and melanization of foreign organisms as well as wound healing. Here, we characterize phenoloxidase extracted from the hemolymph of the cotton leaf worm, Spodoptera littoralis, for the first time. We obtained a cDNA sequence of 600 bp as a new record, which has elevated homology with known PO sequences of other lepidopterous insects. Moreover, we evaluated the antibacterial activity of PO induced by synthesized antimicrobial peptides (AMPs) against Gram-positive Staphylococcus aureus and Enterococci,and Gram-negative Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumonia. Standardized concentrations (0.5 McFarland) of bacterial suspensions were used. The highest antibacterial activity expressed as the “growth-inhibition zone," was 2.0 ± 0.2 Cm observed against Staphylococcus. For the Gram-negative bacteria, a growth-inhibition zone (1.5 ± 0.15 Cm) was observed only for E. coli. Based on these results, future studies evaluating the effect of AMPs compared with existing antibiotics are warranted.

DOI

10.21608/eajbsc.2022.261502

Keywords

antimicrobial peptides, Phenoloxidase, Spodoptera littoralis

Authors

First Name

Doaa

Last Name

Soliman

MiddleName

E.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Cairo11566, Egypt

Email

doaa_soliman@sci.asu.edu.eg

City

-

Orcid

-

First Name

Hanan

Last Name

Amer

MiddleName

S.

Affiliation

Ain Shams University Specialized Hospital. Infectious Control Unit, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Nadia

Last Name

Lotfy

MiddleName

M.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Cairo11566, Egypt

Email

prof_nadia@sci.asu.edu.eg

City

-

Orcid

-

First Name

Wael,

Last Name

Abdel-Mageed

MiddleName

S.,

Affiliation

Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Sadat City, Egypt, 32897 - Genetics Department, Faculty of Agriculture, Beni-Suef University, Beni-Suef, Egypt, 62511

Email

-

City

-

Orcid

-

First Name

Shaimaa

Last Name

Mo'men

MiddleName

A. A.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Cairo11566, Egypt

Email

shaimaa_momen@sci.asu.edu.eg

City

-

Orcid

-

Volume

14

Article Issue

2

Related Issue

35452

Issue Date

2022-12-01

Receive Date

2022-07-27

Publish Date

2022-09-30

Page Start

153

Page End

162

Print ISSN

2090-0767

Online ISSN

2090-083X

Article File

EAJBSC_Volume 14_Issue 2_Pages 153-162.pdf

PDF . 669.4kB

Link

https://eajbsc.journals.ekb.eg/article_261502.html

Detail API

https://eajbsc.journals.ekb.eg/service?article_code=261502

Order

15

Type

Original Article

Type Code

673

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Publication Link

https://eajbsc.journals.ekb.eg/

MainTitle

Molecular Characterization and Evaluation of The Antimicrobial Activity of Phenoloxidase Purified from Spodoptera littoralis against Different Array of Pathogenic Bacteria

Details

Type

Article

Created At

22 Jan 2023