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260478

Simplification of the Culture Medium and Action of The Bioreactor Material on The Stimulation of Chlorella vulgaris Production

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Last updated: 03 Jan 2025

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Abstract

Cultivation Chlorella vulgaris still remains a challenge for scientists against the background of the cultural systems and the different cultural media deployed. In this context, our study aimed to a comparison of culture media BG11 known for its validity in the culture and new media GS1 and GS2 (rich in ammoniacal and nitric nitrogen) easy to prepare and used. Further culture parameters were involved such as photoperiod (16 h and 24 h) and the materials of the culture contents used in the bioreactor (glass bottles, plastic bottles in LDPE (low-density polyethylene) and plastic bottles in PET (polyethylene terephthalate)). The obtained results indicated that these parameters displayed a direct effect on the productivity of the culture and that their effect was mutual. However, for optimal production of C.vulgaris biomass, it is recommended to use a GS1 culture medium at a photoperiod of 24h, for a production of 50.6 x 106 cells/ml, and fresh biomass of 0.35 g/L. It is however advised to respect the different types' rates of nitrogen in order to avoid cell division inhibition. Therefore, a concentration of 0.13 g/L of nitric nitrogen and 0.28 g/ L of ammoniacal nitrogen is required, with a low dose of urea nitrogen of 0.34g/ L.
              The use of PET plastic in the bioreactor restricts the adhesion of the culture to the cylinders' walls, and with reduced loss rate, a homogeneous diffusion of the light in the totality of the cultures, and a lifetime of the bioreactor remains greater.

DOI

10.21608/eajbsc.2022.260478

Keywords

Chlorella vulgaris, cultural medium, Photoperiod, Biomass, Bioreactor

Authors

First Name

Ghomari,

Last Name

samia

MiddleName

-

Affiliation

Laboratoire de Nutrition, Pathologie, Agro-Biotechnologie et santé. Djillali Liabès university, Natural and Life Sciences faculty, BP 89, 22000 Sidi-Bel-Abbès, Sidi-Bel-Abbès, Algéria.

Email

samiabiotech73@yahoo.fr

City

Sidi Bel Abbès

Orcid

-

First Name

Benabdesslem,

Last Name

Yasmina

MiddleName

-

Affiliation

Laboratoire de Nutrition, Pathologie, Agro-Biotechnologie et santé. Taher Moulay University of Saida. Faculty of Natural and Life Sciences, Algeria.

Email

yasmin46@live.fr

City

Temouchent

Orcid

-

First Name

Mhamdia,

Last Name

Chafik

MiddleName

-

Affiliation

Laboratoire de Nutrition, Pathologie, Agrobiotechnologie et Santé, Faculty of Natural and Life Sciences, Djillali Liabes University, Sidi-bel-Abbes, Algeria.

Email

mhamdiachafik@yahoo.fr

City

Sidi Bel Abbès

Orcid

-

First Name

Daoud Hamdi,

Last Name

samia

MiddleName

-

Affiliation

INRAA-Ouest, Sidi Bel Abbes. Algeria

Email

daoudsamia27@yahoo.fr

City

Sidi Bel Abbès

Orcid

-

First Name

Khaled,

Last Name

Meghit Boumediene

MiddleName

-

Affiliation

Laboratoire de Nutrition, Pathologie, Agrobiotechnologie et Santé, Faculty of Natural and Life Sciences, Djillali Liabes University, Sidi-bel-Abbes, Algeria

Email

khaled@khaledmb.co.uk

City

Sidi Bel Abbès

Orcid

-

First Name

Cherifi,

Last Name

Kouider

MiddleName

-

Affiliation

Laboratoire de Valorisation de la Biodiversité; et Conservation des Plantes, Djillali Liabès University, Faculty of Natural and Life Sciences Sidi-Bel-Abbes, Algeria.

Email

-

City

-

Orcid

-

Volume

14

Article Issue

2

Related Issue

35452

Issue Date

2022-12-01

Receive Date

2022-07-28

Publish Date

2022-09-23

Page Start

85

Page End

99

Print ISSN

2090-0767

Online ISSN

2090-083X

Article File

EAJBSC_Volume 14_Issue 2_Pages 85-99.pdf

PDF . 773.3kB

Link

https://eajbsc.journals.ekb.eg/article_260478.html

Detail API

https://eajbsc.journals.ekb.eg/service?article_code=260478

Order

10

Type

Original Article

Type Code

673

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Publication Link

https://eajbsc.journals.ekb.eg/

MainTitle

Simplification of the Culture Medium and Action of The Bioreactor Material on The Stimulation of Chlorella vulgaris Production

Details

Type

Article

Created At

22 Jan 2023