Responses to antioxidants are reported in this research. Some indigenous plants have catalase (CAT), superoxide dismutase (SOD), and peroxidase activity (POD) as scavengers of reactive oxygen species (ROS) for mitigating environmental circumstances. In May 2019, physicochemical parameters such as EC, TDS, TSS, pH, HCO₃, CO₃-, Cl-, and SO₄ were measured. Five locations in Egypt's Saint Katherine Mountain were chosen. During the sampling process, A statistical analysis of Physico-chemical components discovered considerable variations in numerous chemical and physical soil variables among diverse wadis. Changes in HCO₃ were found to be non-significant, but substantial and highly-significant variations in other chemical and physical property measures were discovered among the five wadis examined. The current study found that in a stressed ecosystem, the plants chosen overcome the stress by altering their stress enzyme activities, implying evidence of adaptive mechanisms to thrive in a stressful environment and the suitability of three plant species—Phlomis aurea, Ballota undulata, and Nepeta Septemcrenata for environmental matrices (as indicators), particularly under altered climatic conditions.