In this study, the total phenolic levels, antioxidant and antibacterial activities of Claladods from Opuntia ficus indica extracts originating from Saida in Algeria were determined. The phenolic components of these extracts were also determined using HPLC. Total phenolic levels ranged from 10.75 to 1.80 mg g EC/mg E; the total flavonoid components of the extract ranged from 7.99 to 1.31 g EC/mg E. Antioxidant activity of the aqueous extract was increased according to FRAPP, CE50 scored 750 2.1 (g/ml) and free radical DPPH antiradicular activity was scored CI50 (18724.3 g/ml). The phenolic compounds identified by HPLC were acid cinapine ascorbic acid nicotamide tannic acid caffein vanillin kaempherol quercetin myirecetinepicatechin/catechin and five unknown compounds. All of the plants studied contain kaempherol (41%), epicatechin/catechin (14.5%), quercetin (12.9%), myrecetin (8, 9%), Vaniline (7.8%), Ascorbic Acid (3.1%), Nicotamide (2.7%), Tannic Acid (0.4%), Caffeine (0.1%), Cinapic Acid (0.1 %). the aqueous extract has significant antioxidant activities in vitro, the results indicated that the OFI could be attributed to a potential source of natural antioxidant for food applications, The antibacterial activity of Opuntia ficus were estimated towards multiple types of bacteria (Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, and Bacillus Subtilis). The plant shows high activity against all types of bacteria. The obtained results of the antibacterial application inhibitors seem to be quite promising material in the antibacterial fields.