Fish is an extremely important factor in human food and health, and fish varies in its environment from one species to another. Therefore, the aim of this study was to find out whether this environmental difference had an effect on the difference in the biochemical (food) component of these fish? ; This was done by selecting two types of fish that differ from each other in their aquatic environment in terms of the nature of the water and depth (Oreochromis niloticus from the freshwater - the Nile River, and the Solea solea from the marine water beds - Suez Gulf) and the approximate composition and components were analyzed seasonally biochemical in the muscle tissue of these two species, the amount of crude protein, fat, carbohydrate, calorific value, moisture and ash were measured. The results showed that despite the apparent difference in the values ​​of the quantitative biochemical analysis, the statistical analysis reported that there were no significant differences between the two environmentally different species in the values ​​of protein, fat and water content, where P values <0.05; While carbohydrates, calorific value and ash values ​​recorded clear significant differences between the two species with environmental differences where P values >0.05, which means that the difference may be qualitative rather than quantitative, and this was confirmed by the qualitative analysis through the application of protein electrophoresis technology as an example, which showed a clear qualitative discrepancy between the two types under study.