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133373

Study of UGT1A1 Gene Mutation in People Suspected of Hyperbilirubinemia and Its Relationship with Fatty Liver Syndrome by Tetra ARMS-PCR Technique

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Last updated: 03 Jan 2025

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Abstract

Hyperbilirubinemia is one of the most common disorders in the world. The disease has spread out the Middle East to Africa. The importance of this disease is high due to its widespread prevalence in the world. In this study, to investigate hyperbilirubinemia and the difference between the mutations in the UGTA1 gene with hyperbilirubinemia and the fatty liver syndrome was selected from the SNPs involved in expressing the UGTA1 gene named rs372326047.DNA genomic was extracted and one pair of primer for amplifying of the rs372326047 was designed with the help of the Oligo Version 7.0.  After the PCR reaction, samples were sequenced to determine of the fragment of UGTA1 gene.  Figures 1 and 2 showed the sequences were analyzed using the Blast program. Results observed that no mutation between UGTA1 gene. In Table 1. Ten cases from forty samples were selected including, healthy, carrier, sick and different parameters that were questioned from them. Patients have been heart diseases, diabetic-hepatic, and some patients did not have a history of inheritance diseases in family, rate of Hb in some cases was high (more than ten). Most of the cases also had high anemia and regarding some cases, the history of hyperbilirubinemia and its relationship with fatty liver diseases in family was positive and some cases was negative. Table 2. One-way ANOVA followed by Tuckey's post hoc test and HWE frequency of allelic and genotyping of nucleotide of TT, TC and CC of rs372326047of UGTA1 gene in cases and normal population respectively. Therefore, the results of this study show that there is no relationship between mutations in the rs372326047 of UGTA1 gene in hyperbilirubinemia and its relationship with fatty liver syndrome.

DOI

10.21608/eajbsz.2020.133373

Keywords

Hyperbilirubinemia, Fatty liver diseases, Mutation

Authors

First Name

Ali

Last Name

Janbaz

MiddleName

-

Affiliation

M.Sc in Genetics, Department of Biological Sciences, Islamic Azad University, Tonekabon Branch, IRAN

Email

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City

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Orcid

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First Name

Abolhasan

Last Name

Rezaei

MiddleName

-

Affiliation

Department of Biological Sciences, Faculty of Genetics, Islamic Azad University, Tonekabon Branch, IRAN

Email

a.rezaei@tonekaboniau.ac.ir

City

-

Orcid

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Volume

12

Article Issue

2

Related Issue

15675

Issue Date

2020-12-01

Receive Date

2020-09-30

Publish Date

2020-12-01

Page Start

205

Page End

213

Print ISSN

2090-0759

Online ISSN

2090-0821

Article File

EAJBSZ_Volume 12_Issue 2_Pages 205-213.pdf

PDF . 913.1kB

Link

https://eajbsz.journals.ekb.eg/article_133373.html

Detail API

https://eajbsz.journals.ekb.eg/service?article_code=133373

Order

18

Type

Original Article

Type Code

672

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, B. Zoology

Publication Link

https://eajbsz.journals.ekb.eg/

MainTitle

Study of UGT1A1 Gene Mutation in People Suspected of Hyperbilirubinemia and Its Relationship with Fatty Liver Syndrome by Tetra ARMS-PCR Technique

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Article

Created At

22 Jan 2023