Fruit peels annually accumulate in huge amounts, fruit wastes are rich in lignocellulo-sic component which can be recovered into mono simple carbohydrates able to utilize for bioethanol production. The enzymatic hydrol-ysis of lignocelluloses is known to be a key to the second-generation biofuel, the challenge is the still expensive enzymes involved in the saccharification process, loss of the most hem-icellulose pentose sugars which were non-fer-mentable as base, beside the presence of ham-pers lignin thus require to resolve its problem.
As part of study, followed the cost-effective means for bioethanol producing from four fruit peels (Banana, Orange, Mango and Water-melon), yielding of enzymes from co-fungal cultivation carried by Aspergillus niger and Phanerochaete chrysosporium, followed by co-fermentation of the saccharified sugars us-ing yeast belong to Saccharomyces cerevisiae and Kluyveromyces marxianus which contrib-uted for bioethanol production in 8 L lab-scale reactor. The fermented sugars recovering was reached to 27.77 g.l-1 from banana peels which were found to be good exploited as potential raw source, co-fungal enzymatic hydrolysis followed by yeast co-fermentation led to sub-stantial yield by 10.74 g.l-1, the adding of calcium oxide increased the purity which leads finally to 97.5 wt % of pure bioethanol. Thrust towards fossil fuels replacement with renewa-ble clean fuels such as bioethanol by using fruit peels residuals which are considered renewa-ble energy source may also help in CO2 miti-gation.