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153541

ISOLATION AND IDENTIFICATION OF ANTIBIOTIC PRODUCING PSEUDOMONAS FLUORESCENS NBRC-14160 FROM DELTA SOIL IN EGYPT

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Last updated: 03 Jan 2025

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Abstract

Pseudomonas fluorescens is one of plant Growth Promoting Rhizobacteria (PGPR) members which has a major role in the biological control of bacterial and fungal pathogens. A research was conducted at the Laboratory of Microbiology, Faculty of Agriculture, Ain Shams University, Qalubyia governorate intended for isolating Pseudomonas fluorescens isolate, efficient in antibiotic production. For isolation, soil samples, collected from Faculty of agriculture farm soil at depth of 10 cm, were screened for Ps. fluorescens isolates by cultivating the samples on King's medium. Out of 30 isolates obtained, one was selected based on its high lipid content, because of the lipid's correlation to antibiotic production and inhibitory activity. The selected isolate was characterized by morphological, physiological, biochemical tests then confirmed its identity by 16S rRNA gene sequence analysis and named Pseudomonas fluorescens NBRC-14160 16srRNA. Morphological features showed that Ps. fluorescens NBRC-14160 has large colonies, with irregular surface, opaque, producing green fluorescent pigments. Cells are short rods, Gram negative. Physiological features indicated that Ps. fluorescens NBRC-14160 is capable of producing several exoenzymes including lipase, phospholipase, protease and chitinase and incapable of producing amylase and cellulase. Carbohydrate fermentation tests were positive for fructose, glucose, D-glucose, and galactose, forming acids after 24 hrs. of incubation at 30˚C. However, it loses the ability to ferment inositol, mannose, xylose, mannitol, raffinose and rhamnose sugars. It's lipid content was 607 mg/g. Inhibitory activity was studied by the method of disc diffusion test against nine pathogenic bacterial and fungal strains. Staphylococcus aureus was the most sensitive bacterial pathogen towards P. fluorescens NBRC-14160 with a 4.5 cm zone of inhibition, while Serratia marscens had 0.95 cm zone. Aspergillus niger and Alternaria solani were the most sensitive fungal pathogens towards P. fluorescens NBRC-14160 with 2.55 and 2.5 cm of inhibition zone, respectively. However, the most resistant fungus was Fusarium oxysporum with 1.3 cm inhibition zone.

DOI

10.21608/ajs.2020.153541

Keywords

Pseudomonas fluorescens, physiological characteristics, exo-enzymes, production, antimicrobial activity, 16S rRNA

Authors

First Name

Mahmoud

Last Name

Yahia

MiddleName

-

Affiliation

Agricultural Microbiology Dept, Faculty of Agriculture, Ain Shams Univ., P.O. Box 68 Hadyek Shoubra 11241, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Mohamed

MiddleName

-

Affiliation

Agricultural Microbiology Dept, Faculty of Agriculture, Ain Shams Univ., P.O. Box 68 Hadyek Shoubra 11241, Cairo, Egypt

Email

mohamedhashem10@icloud.com

City

-

Orcid

0000-0001-6496-3357

First Name

Mostafa

Last Name

Othman

MiddleName

-

Affiliation

Agricultural Microbiology Dept, Faculty of Agriculture, Ain Shams Univ., P.O. Box 68 Hadyek Shoubra 11241, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Doaa

Last Name

Mostafa

MiddleName

-

Affiliation

Agricultural Microbiology Dept, Faculty of Agriculture, Ain Shams Univ., P.O. Box 68 Hadyek Shoubra 11241, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Gomaa

MiddleName

-

Affiliation

Agricultural Microbiology Dept, Faculty of Agriculture, Ain Shams Univ., P.O. Box 68 Hadyek Shoubra 11241, Cairo, Egypt

Email

mohamedahmed_gomaa@agr.asu.edu.eg

City

-

Orcid

0000-0001-5879-4470

First Name

Mohamed

Last Name

Fahmy

MiddleName

-

Affiliation

Agricultural Microbiology Dept, Faculty of Agriculture, Ain Shams Univ., P.O. Box 68 Hadyek Shoubra 11241, Cairo, Egypt

Email

mohamed_fahmy@agr.asu.edu.eg

City

-

Orcid

0000-0002-5184-6095

First Name

Romisaa

Last Name

Shaban

MiddleName

-

Affiliation

Agricultural Microbiology Dept, Faculty of Agriculture, Ain Shams Univ., P.O. Box 68 Hadyek Shoubra 11241, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Kamel

MiddleName

-

Affiliation

Agricultural Microbiology Dept, Faculty of Agriculture, Ain Shams Univ., P.O. Box 68 Hadyek Shoubra 11241, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Mostafa

Last Name

Abdelhai

MiddleName

-

Affiliation

Agricultural Microbiology Dept, Faculty of Agriculture, Ain Shams Univ., P.O. Box 68 Hadyek Shoubra 11241, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Wafaa

Last Name

Radwan

MiddleName

-

Affiliation

Agricultural Microbiology Dept, Faculty of Agriculture, Ain Shams Univ., P.O. Box 68 Hadyek Shoubra 11241, Cairo, Egypt

Email

wafaahamdy57@yahoo.com

City

-

Orcid

0000-0003-3690-7803

First Name

Shawki

Last Name

Selim

MiddleName

-

Affiliation

Agricultural Microbiology Dept, Faculty of Agriculture, Ain Shams Univ., P.O. Box 68 Hadyek Shoubra 11241, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Samah

Last Name

Abu-Hussien

MiddleName

-

Affiliation

Agricultural Microbiology Dept, Faculty of Agriculture, Ain Shams Univ., P.O. Box 68 Hadyek Shoubra 11241, Cairo, Egypt

Email

samah_hashem1@agr.asu.edu.eg

City

-

Orcid

0000-0002-7224-1212

Volume

28

Article Issue

3

Related Issue

22463

Issue Date

2020-09-01

Receive Date

2020-01-09

Publish Date

2020-09-30

Page Start

797

Page End

808

Print ISSN

1110-2675

Online ISSN

2636-3585

Article File

AJS_Volume 28_Issue 3_Pages 797-808.pdf

PDF . 1.0MB

Link

https://ajs.journals.ekb.eg/article_153541.html

Detail API

https://ajs.journals.ekb.eg/service?article_code=153541

Order

7

Type

Original Article

Type Code

668

Publication Type

Journal

Publication Title

Arab Universities Journal of Agricultural Sciences

Publication Link

https://ajs.journals.ekb.eg/

MainTitle

ISOLATION AND IDENTIFICATION OF ANTIBIOTIC PRODUCING PSEUDOMONAS FLUORESCENS NBRC-14160 FROM DELTA SOIL IN EGYPT

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Article

Created At

22 Jan 2023