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35617

ISOLATION AND MOLECULAR IDENTIFICATION OF INDIGENOUS BACTERIAL ISOLATES ABLE TO DEGRADE ORGANOPHOSPHATES

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Last updated: 03 Jan 2025

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Abstract

The wide and indiscriminate use of pesticides for pest control in agriculture has inflicted serious harm and problems to humans as well as to the biodiversity. Microbial degradation of pesticides in contaminated soils has been considered advantageous to decontaminate areas that have been polluted by pesticides. Chlorpyrifos and diazinon were the most persistent residues in Egyptian soils. Four bacterial isolates were isolated from organophosphorus insecticides contaminated soils and genetically identified based on DNA sequence of 16s rDNA gene, Cronobacter muytjensii GH10, Achromobacter xylosoxidans GH9OP, Pseudomonas aeruginosa GH2NO8 and Pseudomonas putida GH4SNO/P were able to degrade 92.59%, 97.75%, 91.82%, and 90.78% of diazinon (600mg/l) as compared with 16.99% in control and 93.43%, 78.51%, 93.18% and 95.36% of chlorpyrifos (480mg/l) as compared with 4.28%, in control, respectively after 20 days of incubation.  
 

DOI

10.21608/ajs.2018.35617

Keywords

Organophosphates, biodegradation, 16sr DNA gene, Gas chromatography analysis

Authors

First Name

Ghada

Last Name

El-sayed

MiddleName

M.

Affiliation

Microbial Genetics Dept., National Research Centre., P.O. Box 12622, 33 El-Bohouth St., Dokki, Giza, Egypt

Email

ghada.khalefa@yahoo.com

City

-

Orcid

-

First Name

S.

Last Name

Ibrahim,

MiddleName

A.

Affiliation

Genetic. Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68, Hadayek Shubra 11241, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Nivien

Last Name

Abosereh

MiddleName

A.

Affiliation

Microbial Genetics Dept., National Research Centre., P.O. Box 12622, 33 El-Bohouth St., Dokki, Giza, Egypt

Email

-

City

-

Orcid

-

First Name

A.

Last Name

Abd El-Razik

MiddleName

B.

Affiliation

Genetic. Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68, Hadayek Shubra 11241, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Fatma

Last Name

Hafez

MiddleName

M.

Affiliation

Microbial Genetics Dept., National Research Centre., P.O. Box 12622, 33 El-Bohouth St., Dokki, Giza, Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Hammad

MiddleName

A.

Affiliation

Plant Protection Dept., Fac. of Agric., Ain Shams Univ., P.O. Box 68, Hadayek Shobra 11241, Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

26

Article Issue

Special issue (2D)

Related Issue

5831

Issue Date

2018-02-01

Receive Date

2018-08-09

Publish Date

2018-02-01

Page Start

2,503

Page End

2,511

Print ISSN

1110-2675

Online ISSN

2636-3585

Article File

AJS_Volume 26_Issue Special issue (2D)_Pages 2503-2511.pdf

PDF . 1.1MB

Link

https://ajs.journals.ekb.eg/article_35617.html

Detail API

https://ajs.journals.ekb.eg/service?article_code=35617

Order

34

Type

Original Article

Type Code

668

Publication Type

Journal

Publication Title

Arab Universities Journal of Agricultural Sciences

Publication Link

https://ajs.journals.ekb.eg/

MainTitle

ISOLATION AND MOLECULAR IDENTIFICATION OF INDIGENOUS BACTERIAL ISOLATES ABLE TO DEGRADE ORGANOPHOSPHATES

Details

Type

Article

Created At

22 Jan 2023