The aim of this study was biodiesel purification with different methods beyond water by using different materials was used for this purpose. A large amount of biodiesel was produced from canola oil as source of oil therefore methanol alcohol had been added with potassium hydroxide (KOH) as catalyst. After 8 hours, biodiesel was separated from glycerol where glycerol was under the biodiesel using gravity. To purify biodiesel from remains of glycerol, water and alcohol, some materials filled two filters. Materials were rice husk grind, bagasse grind, saw dust, magnesium silicate and water. The results showed that the water, methanol and glycerol contents were 0.04, 0.01 and 0.23% respectively with using grind bagasse, but they were 0.2, 0.03 and 0.24% respectively with using water in purification process. So the bagasse grind was better than other materials in purification process of canola biodiesel.