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15360

IN VITRO PROPAGATION OF SOMATIC EMBRYOGENESIS-DERIVED PLANTLETS OF CITRUS

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Last updated: 03 Jan 2025

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Abstract

Nodal stem segments of in vitro growing somatic embyogenesis and seedling-derived plantlets of sweet orange, C. sinensis (L.) of (Washington navel, shamouti and blood orange cvs.); grapefruit, C. paradisi (L.); Local mandarin, C. deliciosa (Tenor); lemon, C. limon; (L.); citron, C. medica (L.); lime, C. aurantifolia (Christm); rough lemon, C. jambhiri (Lush) and sour orange, C. aurantium (L.) were cultured on MS medium supplemented with different concentrations of BA (0.5,1.0 and 2.0 mg/L) alone or in combination with NAA (0.5 mg/L) for shoot multiplica-tion. The results indicated that somatic embryogenesis-derived plantlets can be suc-cessfully propagated in vitro with a good vigor compared with germinated seedlings. A large number of proliferated shoots were produced when these explants were cul-tured on MS medium containing 0.5mg/L of BA combined with 0.5mg/L of NAA. Most obtained shoots were rooted on half-strength MS medium containing 0.5 mg/L of NAA. These in vitro grown plantlets were then successfully transferred to green house through acclimatization process.

DOI

10.21608/ajs.2006.15360

Keywords

citrus, In vitro propagation, Nodal segments, multiplication, Rooting

Authors

First Name

El-Sawy, A.

Last Name

A.

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-

Affiliation

Plant Biotechnology Dep., Gen.Eng. and Bio. Div., National Research Center, Dokki, Egypt.

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First Name

A.

Last Name

Reda

MiddleName

-

Affiliation

Plant Biotechnology Dep., Gen.Eng. and Bio. Div., National Research Center, Dokki, Egypt.

Email

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City

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Orcid

-

First Name

A.

Last Name

Gomaa

MiddleName

-

Affiliation

Pomology Dep., Faculty of Agriculture, Cairo University, Giza, Egypt.

Email

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City

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Orcid

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First Name

Nansy

Last Name

Danial

MiddleName

-

Affiliation

Pomology Dep., Faculty of Agriculture, Cairo University, Giza, Egypt.

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Orcid

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Volume

14

Article Issue

2

Related Issue

3244

Issue Date

2006-09-01

Receive Date

2018-09-30

Publish Date

2006-09-01

Page Start

723

Page End

740

Print ISSN

1110-2675

Online ISSN

2636-3585

Link

https://ajs.journals.ekb.eg/article_15360.html

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https://ajs.journals.ekb.eg/service?article_code=15360

Order

12

Type

Original Article

Type Code

668

Publication Type

Journal

Publication Title

Arab Universities Journal of Agricultural Sciences

Publication Link

https://ajs.journals.ekb.eg/

MainTitle

IN VITRO PROPAGATION OF SOMATIC EMBRYOGENESIS-DERIVED PLANTLETS OF CITRUS

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Article

Created At

22 Jan 2023