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IDENTIFICATION OF EIGHT HALOTOLERANT STREPTO-MYCETE ISOLATES USING A SUGGESTED NUMERICAL TAXONOMY

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Last updated: 03 Jan 2025

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Abstract

A numerical method was suggested for the identification of eight local halotolerant streptomycete isolates. Cultural, morphological, physiological and antagonistic characteristics of those isolates were determined. Arbitrary scoring of these characters for the eight unknown isolates and those of the more related known Streptomyces species in the keyof Pridham and Tresner (1974) was given and resulted 58 characteristic units. The degree of similarity was determined using the Dice coefficient method and clustering was achieved using the unweighted pair group method average (UPGMA) algorithm. Using this numerical method, six out of the eight Streptomyces isolates, i.e., ST08, ST09, ST11, ST13, ST14 and ST15 were identified as S. longisporus, S. janthinus, S. griseochromogenes, S. antibioticus S. baarnensis and S. albolongus with 100, 95.8, 95.0, 92.8, 95.0 and 91.1%, respectively. Isolates ST10 and ST12 were duplicate of S. echinatus with similarities of 93.3 and 94.6%, respectively. Application of the suggested numerical taxonomy on the 14 known Streptomyces species revealed that these species fell into three major clusters based on their color of aerial mycelia.

DOI

10.21608/ajs.2005.15265

Keywords

Streptomyces, Numerical identification, Halotolerant, Characteristic units, Taxonomy

Authors

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H.

Last Name

Mohamed Sonya,

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Affiliation

Agricultural Microbiology Department, Institute of Soil, Water and Environment Research, Agricultural Research Center, Giza, Egypt

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First Name

Saleh

Last Name

E.A.

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Affiliation

Agricultural Microbiology Department, Institute of Soil, Water and Environment Research, Agricultural Research Center, Giza, Egypt

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First Name

Zaki

Last Name

M.M.

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-

Affiliation

Agricultural Microbiology Department, Faculty of Agriculture, Ain Shams Universi-ty, Shoubra El-Kheima, Cairo, Egypt

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Volume

13

Article Issue

3

Related Issue

3215

Issue Date

2005-09-01

Receive Date

2005-04-16

Publish Date

2005-09-01

Page Start

641

Page End

668

Print ISSN

1110-2675

Online ISSN

2636-3585

Link

https://ajs.journals.ekb.eg/article_15265.html

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https://ajs.journals.ekb.eg/service?article_code=15265

Order

5

Type

Original Article

Type Code

668

Publication Type

Journal

Publication Title

Arab Universities Journal of Agricultural Sciences

Publication Link

https://ajs.journals.ekb.eg/

MainTitle

IDENTIFICATION OF EIGHT HALOTOLERANT STREPTO-MYCETE ISOLATES USING A SUGGESTED NUMERICAL TAXONOMY

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Article

Created At

22 Jan 2023