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15263

ISOLATION AND IDENTIFICATION OF Spiroplasma citri USING SOME SENSITIVE METHODS

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Last updated: 03 Jan 2025

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Abstract

          Spiroplasma citri, the causal agent of stubborn disease, was isolated from leaves and fruits of diseased citrus plants and cultured on solid and liquid C3G medium. On the basis of mode of transmission, symptomatology, shape on solid medium, staining of infected plant tissues with Dienes' stain and phase contrast microscopy, the isolated agent was identified as Spiroplasma citri. Identifiction was ensured by ELISA and PCR techniques. A fragment (1053 bp) from the spiralin gene of S. citri was amplified by PCR using two specific primers for the spiralin gene. The nucleic acid hybridization techniques (Southern and dot blots) were used for identification of the spiroplasmal genome using non-radioactive DNA probe specific for spiralin gene region. It was carried out as an alternative sensitive method for rapid detection of the Egyption isolate of S. citri   using non-radioactive DNA probe specific for spiralin gene region.

DOI

10.21608/ajs.2005.15263

Keywords

Spiroplasma citri, Spiralin gene, PCR, DNA hybridization

Authors

First Name

Mahrous

Last Name

Rasha M.

MiddleName

-

Affiliation

Virus and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Research Center, (ARC), P.O. Box 12619, Giza, Egypt

Email

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City

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Orcid

-

First Name

Moustafa

Last Name

T.A.

MiddleName

-

Affiliation

Virus and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Research Center, (ARC), P.O. Box 12619, Giza, Egypt

Email

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City

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Orcid

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First Name

Galal Farag

Last Name

Azza

MiddleName

-

Affiliation

Plant Pathology Dept., Faculty of Agriculture, Ain Shams University; P.O. Box 68 Hadayek Shobra, 1241, Shubra El-Kheima, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Sadik

Last Name

A.S.

MiddleName

-

Affiliation

Plant Pathology Dept., Faculty of Agriculture, Ain Shams University; P.O. Box 68 Hadayek Shobra, 1241, Shubra El-Kheima, Cairo, Egypt

Email

-

City

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Orcid

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First Name

El-Afifi

Last Name

Sohair I.

MiddleName

-

Affiliation

Agric. Microbiology Department, Faculty of Agriculture, Ain Shams University; P.O. Box 68 Hadayek Shobra,1241, Shubra El-Kheima, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Gamal El-Din

Last Name

A.S.

MiddleName

-

Affiliation

Agric. Microbiology Department, Faculty of Agriculture, Ain Shams University; P.O. Box 68 Hadayek Shobra,1241, Shubra El-Kheima, Cairo, Egypt

Email

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City

-

Orcid

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Volume

13

Article Issue

3

Related Issue

3215

Issue Date

2005-09-01

Receive Date

2005-05-03

Publish Date

2005-09-01

Page Start

625

Page End

639

Print ISSN

1110-2675

Online ISSN

2636-3585

Article File

AJS_Volume 13_Issue 3_Pages 625-639.pdf

PDF . 211.3kB

Link

https://ajs.journals.ekb.eg/article_15263.html

Detail API

https://ajs.journals.ekb.eg/service?article_code=15263

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Original Article

Type Code

668

Publication Type

Journal

Publication Title

Arab Universities Journal of Agricultural Sciences

Publication Link

https://ajs.journals.ekb.eg/

MainTitle

ISOLATION AND IDENTIFICATION OF Spiroplasma citri USING SOME SENSITIVE METHODS

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Article

Created At

22 Jan 2023