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18135

Cyanamide-Induced Hepatotoxicity and the Potential Protective Role of Pomegranate Seed Extract in Adult Male Albino Rats

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Last updated: 22 Jan 2023

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Abstract

Background: Dormex, which is used as agricultural fertilizer, contains cyanamide as an active ingredient. Pomegranate fruits enclose numerous polyphenols that guard normal cells against oxidative stress. Aim of the work: to study the hepatotoxic effects of Dormex and assess the protective capacity of pomegranate seed extract (PSE) in rats after 3 months of oral administration. Material & methods: Sixty mature male albino rats were distributed to; Group I, II: rats were given 0.1 ml distilled water/kg/day and 400 mg/kg/day of pomegranate seed extract (PSE), respectively. Group III: divided into two subgroups in which rats received 30 & 60 mg/kg/day Dormex, respectively. Group IV: divided into two subgroups in which rats were given the same dose of Dormex plus PSE. Body and liver weights, serum albumin, total bilirubin and liver enzymes were assessed. Hepatic malondialdehyde (MDA), glutathione reductase and catalase activities were evaluated. Liver specimens were studied with H. &E., Mallory's trichrome and caspase-3 immunostaining. Results: The hepatic index, liver enzymes, bilirubin, and MDA were significantly increased (P˂0.001), while albumin, glutathione reductase, and catalase were lowered significantly in rats of experimental groups compared to the control (P˂0.001). All these assessed parameters were within normal range in the protected groups. The histopathological changes included liver inflammation, degeneration and fibrosis, in addition to apoptosis. Treatment with PSE markedly prevented the occurrence of these abnormalities. Conclusion: Cyanamide prompts oxidative stress that compromises the liver function and eventually liver fibrosis results. These toxic effects are dose related. The PSE exhibits hepatoprotective effects and highlights the possibility of its use as a protective agent in individuals at high risk of Dormex toxicity.

DOI

10.21608/ajfm.2018.18135

Keywords

Dormex, Cyanamide, pomegranate, Oxidative Stress, liver enzymes, histopathology

Authors

First Name

Naema

Last Name

Elhosary

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Affiliation

Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine,Tanta University, Tanta, Egypt

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First Name

Doaa Elgharbawy

Last Name

Elgharbawy

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Affiliation

Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine,Tanta University, Tanta, Egypt

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Orcid

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First Name

Amira

Last Name

Kassab

MiddleName

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Affiliation

Department of Histology, Faculty of Medicine,Tanta University, Tanta, Egypt

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First Name

RM

Last Name

Elgharabawy

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-

Affiliation

Department of Pharmacology and Toxicology, Faculty of Pharmacy. Tanta University, Tanta, Egypt.

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Volume

30

Article Issue

1

Related Issue

3772

Issue Date

2018-01-01

Receive Date

2018-11-01

Publish Date

2018-01-01

Page Start

38

Page End

51

Print ISSN

1687-1030

Online ISSN

2636-3356

Link

https://ajfm.journals.ekb.eg/article_18135.html

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https://ajfm.journals.ekb.eg/service?article_code=18135

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5

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Original Article

Type Code

665

Publication Type

Journal

Publication Title

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

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https://ajfm.journals.ekb.eg/

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Article

Created At

22 Jan 2023